Containers and methods for cell transduction

A method for introducing DNA to a target cell via transduction with a viral vector that is a lentivirus vector or a retrovirus vector, the method comprising

• providing a container in the form of a cell culture bag having an outer surface and an inner surface, the inner surface comprising
• a fluorinated ethylene propylene fluoropolymer;
• attached to the fluoropolymer, a plurality of functional groups;
• attached to the fluoropolymer by covalent bonding to at least a first portion of the functional groups, a first nucleotide aptamer sequence having a binding affinity for the viral vector;
• and
• attached to the fluoropolymer by covalent bonding to at least a second portion of the functional groups, a second nucleotide aptamer sequence having a binding affinity for a VLA-4 or VLA-5 cell receptor of the target cell;
• and
• incubating the viral vector and the target cell in the container, such that the viral vector is bound to the first nucleotide aptamer sequence and the target cell is bound to the second nucleotide aptamer sequence.