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US 12,180,305 B2Granted
Systems and methods for immobilizing a target protein
AssigneeUniversity of South Florida
InventorsHouman Yaghoubi, Arash Takshi, John Thomas Beatty
Life of this patent
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Abstract
In some embodiments, a bioelectronic device includes an electrode, target proteins, and attachment mechanisms that immobilize the target proteins on the electrode, the attachment mechanisms comprising linker proteins that interface with the target proteins and attach the target proteins to the electrode.
Claims9
A method for immobilizing a target protein on an electrode, the method comprising:
- providing the electrode comprising a bare metal surface, wherein the bare metal surface is an outermost surface of the electrode;
- attaching a linker protein comprising cytochrome c to the bare metal surface of the electrode by directly attaching the linker protein comprising cytochrome c to the bare metal surface of the electrode;
- and
- directly attaching the target protein to the attached linker protein to immobilize the target protein relative to the bare metal surface of the electrode,
- wherein the target protein is only indirectly attached to the bare metal surface of the electrode via the linker protein.
2 more elements
A method for immobilizing a target protein on an electrode, the method comprising:
- providing the electrode comprising a bare metal surface, wherein the bare metal surface is an outermost surface of the electrode;
- attaching a linker protein comprising plastocyanin to the bare metal surface of the electrode by directly attaching the linker protein comprising plastocyanin to the bare metal surface of the electrode;
- and
- directly attaching the target protein to the attached linker protein to immobilize the target protein relative to the bare metal surface of the electrode,
- wherein the target protein is only indirectly attached to the bare metal surface of the electrode via the linker protein.
2 more elements
A method for immobilizing a target protein on an electrode, the method comprising:
- providing the electrode comprising a bare metal surface, wherein the bare metal surface is an outermost surface of the electrode;
- attaching a linker protein to the bare metal surface of the electrode by directly attaching the linker protein to the bare metal surface of the electrode;
- and
- directly attaching the target protein to the attached linker protein to immobilize the target protein relative to the bare metal surface of the electrode,
- wherein the target protein is only indirectly attached to the bare metal surface of the electrode via the linker protein, and
- wherein directly attaching the target protein to the attached linker protein further comprises:
- directly attaching at least one of R. sphaeroides bacterium RC, R. sphaeroides bacterium RCLH1 +Pufx, R. sphaeroides bacterium RC-LH1 −Pufx, a Photosystem I protein, a Photosystem II protein, a protein complex from the phycobilisome protein family, or a bacteriorhodopsin protein to the attached linker protein.
4 more elements
Dependencies are inferred from the claim text.