Antibodies binding human and monkey CD3 and uses thereof
AssigneeBeijing Mabworks Biotech Co., Ltd.
InventorsJiangmei Li, Fangjie Liu, Chunyang Jin, Yu Liu, Sijia Huang, Feng Li
Life of this patent
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Abstract
Disclosed is a monoclonal antibody that binds human and monkey CD3, or an antigen-binding portion thereof, as well as the use of the antibody or antigen-binding portion thereof in treatment of inflammatory diseases and in preparation of bispecific antibodies. A bispecific antibody against e.g., CD3 and CD20 comprising the antibody or antigen-binding portion thereof of the disclosure, and the use of the bispecific antibody in treatment of diseases such as cancers are also provided.
Claims17
An isolated monoclonal antibody, or an antigen-binding portion thereof, capable of binding to CD3, comprising a heavy chain variable region comprising a VH CDR1 region, a VH CDR2 region and a VH CDR3 region, and a light chain variable region comprising a VL CDR1 region, a VL CDR2 region and a VL CDR3 region, wherein the VH CDR1 region comprises the amino acid sequence X1YAMN (SEQ ID NO:
- 1), wherein X1 is Thr (T),
- the VH CDR2 region comprises the amino acid sequence RIRSKYNNYATYYAX1SV (SEQ ID NO:2), wherein X1 is Be (I),
- the VH CDR3 region comprises the amino acid sequence HGNFGNSYX1SX2WAY (SEQ ID NO:
- 3), wherein X1 and X2 are Leu (L) and Tyr (Y), respectively,
- the VL CDR1 region comprises the amino acid sequence X1SSTGAVTTX2NYAN (SEQ ID NO:
- 4), wherein X1 and X2 are Gln (Q) and Asn (N), respectively,
- the VL CDR2 region comprises the amino acid sequence GTX1X2X3AP (SEQ ID NO:
- 5), wherein X1, X2 and X3 are Lys (K), Gln (Q) and Arg (R), respectively, and
- the VL CDR3 region comprises the amino acid sequence X1LWYSNLWV (SEQ ID NO:
- 6), wherein X1 is Val (V).
An isolated monoclonal antibody, or an antigen-binding portion thereof, capable of binding to CD3, comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region and the light chain variable region comprise the amino acid sequences
- (1) EVKLLESGGGLVQPKGSLKLSCAASGFTFNTYAMNWVRQAPGKGLEWVARI RSKYNNYATYYAISVKDRFTISRDDSQSILYLQMNNLKTEDTAMYYCVRHGNFGNSYL SYWAYWGQGTLVTVSA (SEQ ID NO:
- 8) and QAVVTQESALTTSPGETVTLTCQSSTGAV TTNNYANWVQEKPDHLFTGLIGGTKQRAPGVPARFSGSLIGDKAALTITGAQTEDEAIYF CVLWYSNLWVFGGGTKLTVL (SEQ ID NO:
- 16), respectively;
- (2) EVQLLESGGGLVQPGGSLRLSCAASGFTFNTYAMNWVRQAPGKGLEWVARI RSKYNNYATYYAISVKDRFTISRDNSKNTLYLQMNSLRAEDTAVYYCVKHGNFGNSYL SYWAYWGQGTLVTVSS (SEQ ID NO:
- 9) and QTVVTQEPSLTVSPGGTVTLTCQSSTGAV TTNNYANWVQQKPGQAPRALIGGTKQRAPGTPARFSGSLLGGKAALTLSGVQPEDEAE YYCVLWYSNLWVFGGGTKLTVL (SEQ ID NO:
- 17), respectively;
- (3) EVQLLESGGGLVQPGGSLRLSCAASGFTFNTYAMNWVRQAPGKGLEWVARI RSKYNNYATYYAISVKDRFTISRDNSKNTLYLQMNSLRAEDTAVYYCVKHGNFGNSYL SYWAYWGQGTLVTVSS (SEQ ID NO:
- 9) and QAVVTQEPSLTVSPGGTVTLTCQSSTGAV TTNNYANWVQQKPGHAFRGLIGGTKQRAPGVPARFSGSLLGGKAALTLSGVQPEDEAE YYCVLWYSNLWVFGGGTKLTVL(SEQ ID NO:
- 18), respectively;
- (4) EVQLLESGGGLVQPGGSLRLSCAASGFTFNTYAMNWVRQAPGKGLEWVARI RSKYNNYATYYAISVKDRFTISRDDSKNTLYLQMNSLRAEDTAMYYCVRHGNFGNSYL SYWAYWGQGTLVTVSS (SEQ ID NO:
- 10) and QTVVTQEPSLTVSPGGTVTLTCQSSTGA VTTNNYANWVQQKPGQAPRALIGGTKQRAPGTPARFSGSLLGGKAALTLSGVQPEDEA EYYCVLWYSNLWVFGGGTKLTVL (SEQ ID NO:
- 17), respectively;
- or
- (5) EVQLLESGGGLVQPGGSLRLSCAASGFTFNTYAMNWVRQAPGKGLEWVARI RSKYNNYATYYAISVKDRFTISRDDSKNTLYLQMNSLRAEDTAMYYCVRHGNFGNSYL SYWAYWGQGTLVTVSS (SEQ ID NO:
- 10) and QAVVTQEPSLTVSPGGTVTLTCQSSTGA VTTNNYANWVQQKPGHAFRGLIGGTKQRAPGVPARFSGSLLGGKAALTLSGVQPEDEA EYYCVLWYSNLWVFGGGTKLTVL(SEQ ID NO:
- 18), respectively.
Dependencies are inferred from the claim text.