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US 12,180,271 B2Granted
Manufacturing methods for producing anti-TNF antibody compositions
AssigneeJanssen Biotech, Inc.
InventorsKristopher Barnthouse, Subinay Ganguly, Maarten Groeneveld, Manuel Lopez, Jr., Michael Nedved, Kevin D. Smith
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Abstract
The present invention relates to methods of manufacture for producing anti-TNF antibodies, e.g., the anti-TNF antibody a recombinant anti-TNF antibody having a heavy chain (HC) comprising amino acid sequence SEQ ID NO:36 and a light chain (LC) comprising amino acid sequence SEQ ID NO:37 and compositions comprising the recombinant anti-TNF antibody.
Claims12
Anti-TNF antibodies comprising:
- (i) a heavy chain comprising the amino acid sequence of SEQ ID NO:36;
- and (ii) a light chain comprising the amino acid sequence of SEQ ID NO:37, wherein the oligosaccharide profile of the anti-TNF antibodies comprises total neutral oligosaccharide species >99.0% and total charged oligosaccharide species <1.0%.
A method of manufacture for producing anti-TNF antibodies comprising:
- (i) a heavy chain comprising the amino acid sequence of SEQ ID NO:36;
- and (ii) a light chain comprising the amino acid sequence of SEQ ID NO:37, wherein the anti-TNF antibodies are produced by a method of manufacture comprising:
- a. culturing Chinese Hamster Ovary cells (CHO cells) with nucleotides encoding the anti-TNF antibodies;
- b. expressing the anti-TNF antibodies in the CHO cells;
- and,
- c. purifying the anti-TNF antibodies, wherein the oligosaccharide profile of the anti-TNF antibodies comprises total neutral oligosaccharide species >99.0% and total charged oligosaccharide species <1.0%.
3 more elements
A composition comprising anti-TNF antibodies comprising:
- (i) a heavy chain comprising the amino acid sequence of SEQ ID NO:36;
- and (ii) a light chain comprising the amino acid sequence of SEQ ID NO:37, wherein the oligosaccharide profile of the anti-TNF antibodies comprises total neutral oligosaccharide species >99.0% and total charged oligosaccharide species <1.0%.
Dependencies are inferred from the claim text.