Compositions and methods for selective labeling of secondary amines
AssigneeEmory University
InventorsMonika Raj, Ogonna Nwajiobi
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Abstract
This disclosure relates to compositions and methods for selective labeling of N-alkyl lysine, N-alkyl adenosine, proline, N-alkyl cytosine, peptides, nucleic acids, or aliphatic or aromatic secondary amines. In certain embodiments, this disclosure relates to methods of forming triazene labeled compounds comprising contacting a compound containing a secondary amine group with a compound comprising an aromatic group with a diazonium para substituted with an electron rich group under conditions such that a triazene labeled compound is formed at the secondary amine group.
Claims8
A method of forming a triazene labeled peptide or nucleic acid comprising contacting a peptide or nucleic acid comprising a secondary amine group and a primarily amine group with a compound comprising an aromatic group with a diazonium para substituted with an electron rich group under conditions providing a triazene labeled peptide or nucleic acid formed at the secondary amine group;
- wherein a triazene labeled peptide is not formed at the primary amine group;
- and
- wherein the conditions providing a triazene labeled peptide or nucleic acid are in an aqueous solution with a pH of between 6.5 to 7.5.
Dependencies are inferred from the claim text.