Separation and identification of myricetin-3-ogalactoside from casuarina glauca

A process for separation and identification of myricetin-3-O-galactoside comprising:

• providing fresh branchlets of a Casuarina glauca plant;
• shade drying and powdering the fresh branchlets to obtain a Casuarina glauca plant powder;
• soaking 300 g of the Casuarina glauca plant powder with 70% ethyl alcohol to obtain a Casuarina glauca plant extract;
• concentrating the Casuarina glauca plant extract to obtain a residue;
• dissolving the residue to obtain an aqueous mixture;
• mixing and sonicating the aqueous mixture;
• centrifuging the aqueous mixture to obtain a supernatant;
• separating myricetin-3-O-galactoside from the supernatant;
• and
• wherein the soaking step is repeated three times in successive portions of 1 L of the 70% ethyl alcohol.