Method for reagent-specific driving EWoD arrays in microfluidic systems

A method of performing droplet operations on a first plurality of droplets having a first composition and on a second plurality of droplets having a second composition using an active matrix electrowetting on dielectric AM-EWoD system

• comprising a plurality of electrodes configured to manipulate droplets of fluid in a microfluidic space, wherein the plurality of electrodes are pixel electrodes disposed in the active matrix electrowetting on dielectric AM-EWoD system, wherein the pixel electrodes are switched by a transistor matrix comprising a plurality of transistors and wherein each transistor of the plurality of transistors of the transistor matrix is operably connected to a gate line, to a source line, and to the pixel electrodes, the method comprising:
• performing one or more first droplet operations on the first plurality of droplets and performing one or more second droplet operations on the second plurality of droplets in parallel by driving the pixel electrodes with a first drive sequence having a first series of voltage pulses and with a second drive sequence having a second series of voltage pulses wherein the first series of voltage pulses differs from the second series of voltages pulses, and wherein each voltage pulse sequence of the first series of voltage pulses and each voltage pulse sequence of the second series of voltage pulses includes a mix of high voltage pulses and of low voltage pulses and wherein each voltage pulse sequence of the first series of voltage pulses differs in intermittency from each voltage pulse sequence of the second series of voltage pulses.