Microfluidic sequencing device for multiplying and separating molecular chains, and method for separating molecular chains obtained from an amplification reaction

A method for separating molecular chains obtained from an amplification reaction, using a microfluidic sequencing device that has (a) a supply opening configured for supplying biochemical material into the sequencing device;

• (b) at least one microfluidic separation-procedure unit including at least one separation-procedure channel that includes (i) at least one amplification cavity configured to reproduce molecular chains supplied via the supply opening as biochemical material, and (ii) at least one separation unit microfluidically connectable or connected to the at least one amplification cavity and including a multipore material, the at least one separation unit configured to separate nucleic acids from further macromolecular constituents and/or to resolve nucleic acids;
• and a discharge opening configured for discharging nucleic acids resolved in the at least one separation-procedure unit from the sequencing device as biochemical material, the method comprising:
• introducing a liquid for the amplification reaction into at least one amplification cavity of at least one separation-procedure channel of at least one microfluidic separation-procedure unit in order to prepare for an amplification reaction;
• conducting a sealing liquid across the supply opening and the discharge opening in order to seal the supply opening and the discharge opening;
• performing the amplification reaction in order to generate at least one molecular chain and terminating obtained molecular chains in order to generate chain-termination products;
• and
• applying a voltage between the supply opening and the discharge opening in order to fill the at least one separation unit and separating the at least one molecular chain generated in the separation unit in order to obtain chain-termination products.

A computer program which is configured to execute and/or control a microfluidic sequencing device, which has (a) a supply opening configured for supplying biochemical material into the sequencing device;

• (b) at least one microfluidic separation-procedure unit including at least one separation-procedure channel that includes (i) at least one amplification cavity configured to reproduce molecular chains supplied via the supply opening as biochemical material, and (ii) at least one separation unit microfluidically connectable or connected to the at least one amplification cavity and including a multipore material, the at least one separation unit configured to separate nucleic acids from further macromolecular constituents and/or to resolve nucleic acids;
• and a discharge opening configured for discharging nucleic acids resolved in the at least one separation-procedure unit from the sequencing device as biochemical material, so as to execute program instructions stored in memory to operate the microfluidic sequencing device to separate molecular chains obtained from an amplification reaction by:
• introducing a liquid for an amplification reaction into at least one amplification cavity of at least one separation-procedure channel of at least one microfluidic separation-procedure unit in order to prepare for an amplification reaction;
• conducting a sealing liquid across the supply opening and the discharge opening in order to seal the supply opening and the discharge opening;
• performing an amplification reaction in order to generate at least one molecular chain and terminating obtained molecular chains in order to generate chain-termination products;
• and
• applying a voltage between the supply opening and the discharge opening in order to fill the at least one separation unit and separating the at least one molecular chain generated in the separation unit in order to obtain chain-termination products.