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US 12,179,196 B2Granted
Fluidic apparatus and methods useful for chemical and biological reactions
AssigneePacific Biosciences of California, Inc.
InventorsChristopher Kilcoin, Kristin Dills, Rebecca McGinley
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Abstract
Provided herein is a method of performing a nucleic acid sequencing reaction using a sequencing system comprising a reagent cartridge coupled to a detection apparatus, and a pump coupled to the reagent cartridge. The reagent cartridge includes a plurality of reagent reservoirs and a main channel. The detection apparatus includes a flow cell that is coupled to the reagent cartridge when the reagent cartridge interacts transiently with the detection apparatus.
Claims22
A method of performing a nucleic acid sequencing reaction, comprising:
- (a) coupling a reagent cartridge to a detection apparatus;
- wherein the reagent cartridge comprises a plurality of reagent reservoirs and a main channel;
- (b) coupling the reagent cartridge to a pump;
- (c) delivering sequencing reagents from the reagent cartridge to a flow cell, wherein the plurality of reagent reservoirs contains the sequencing reagents, and wherein the sequencing reagents comprise a nucleic acid sample for the sequencing reaction;
- and
- (d) detecting the sequencing reaction in the flow cell using the detection apparatus,
- wherein the pump is coupled to the reagent cartridge separately from the detection apparatus;
- wherein the pump is a separate component from the reagent cartridge and the detection apparatus;
- wherein the reagent cartridge interacts transiently with the detection apparatus;
- and
- wherein the flow cell is an integral component of the detection apparatus such that the flow cell is coupled to the reagent cartridge during coupling of the reagent cartridge to the detection apparatus.
8 more elements
A sequencing system, comprising:
- (a) a reagent cartridge coupled to a detection apparatus;
- wherein the reagent cartridge comprises a plurality of reagent reservoirs and a main channel, and wherein the plurality of reagent reservoirs contain sequencing reagents;
- and
- (b) a pump coupled to the reagent cartridge;
- wherein the pump is fluidically coupled to the reagent cartridge separately from the detection apparatus;
- wherein the pump is a separate component from the reagent cartridge and the detection apparatus;
- wherein the reagent cartridge interacts transiently with the detection apparatus;
- and
- wherein a flow cell is an integral component of the detection apparatus such that the flow cell is coupled to the reagent cartridge during coupling of the reagent cartridge to the detection apparatus;
- wherein the system is configured to deliver sequencing reagents from the reagent cartridge to the flow cell.
7 more elements
Dependencies are inferred from the claim text.