Methods for the production of TCR gamma delta + t cells
AssigneeGammaDelta Therapeutics Ltd
InventorsDiogo Antonio Remechido Anjos, Daniel Vargas Correia, Afonso Rocha Martins De Almeida
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Abstract
The present invention relates to novel methods for the isolation and the selective ex vivo expansion of V32′ TCRy6+ T cells and to their clinical application.
Claims14
A method of modulating an immune response comprising administering an effective amount of TCRγδ+ T cells obtained by a method for expanding Vδ2-TCRγδ+ T cells in a sample comprising:
- (1) culturing cells in the sample in a first culture medium comprising a T cell mitogen and interleukin-4;
- in the absence of interleukin-15, interleukin-2, or interleukin-7;
- and
- (2) culturing the cells obtained in step (1) in a second culture medium comprising a T cell mitogen and interleukin-15, interleukin-2, or interleukin-7, in the absence of interleukin-4, to an animal in need thereof.
A method for treating an infection comprising administering an effective amount of TCRγδ+ T cells obtained by a method for expanding Vδ2-TCRγδ+ T cells in a sample comprising:
- (1) culturing cells in the sample in a first culture medium comprising a T cell mitogen and interleukin-4;
- in the absence of interleukin-15, interleukin-2, or interleukin-7;
- and
- (2) culturing the cells obtained in step (1) in a second culture medium comprising a T cell mitogen and interleukin-15, interleukin-2, or interleukin-7, in the absence of interleukin-4, to an animal in need thereof.
A method for treating cancer comprising administering an effective amount of TCRγδ+ T cells obtained by a method for expanding Vδ2-TCRγδ+ T cells in a sample comprising:
- (1) culturing cells in the sample in a first culture medium comprising a T cell mitogen and interleukin-4;
- in the absence of interleukin-15, interleukin-2, or interleukin-7;
- and
- (2) culturing the cells obtained in step (1) in a second culture medium comprising a T cell mitogen and interleukin-15, interleukin-2, or interleukin-7, in the absence of interleukin-4, to an animal in need thereof.
A method for vaccinating an animal comprising administering an effective amount of TCRγδ+ T cells obtained by a method for expanding Vδ2-TCRγδ+ T cells in a sample comprising:
- (1) culturing cells in the sample in a first culture medium comprising a T cell mitogen and interleukin-4;
- in the absence of interleukin-15, interleukin-2, or interleukin-7;
- and
- (2) culturing the cells obtained in step (1) in a second culture medium comprising a T cell mitogen and interleukin-15, interleukin-2, or interleukin-7, in the absence of interleukin-4, to an animal in need thereof.
Dependencies are inferred from the claim text.