Rice grain with reduced ROS1a activity
AssigneesCommonwealth Scientific and Industrial Research Organisation, Institute of Botany, Chinese Academy of Sciences
InventorsRonald Chun Wai Yu, Crispin Alexander Howitt, Philip John Larkin, Chun-Ming Liu, Xiao-Ba Wu, Jinxin Liu
Life of this patent
Estimating the term…
Abstract
The present invention relates to rice grain with thickened aleurone. Also provided is a rice plant comprising at least one genetic variation which reduces the activity of at least one ROS1a gene in the plant. Grain of the invention, or aleurone therefrom, has improved nutritional properties, and hence is particularly useful for human and animal feed products.
Claims20
A method of producing a rice plant which produces grain comprising an aleurone that is thickened compared to aleurone from a corresponding wild-type grain, the method comprising the steps of
- i) introducing into one or more rice cells a mutation of an endogenous ROS1a gene such that the mutated ROS1a gene encodes a ROS1a polypeptide whose amino acid sequence is different compared to the amino acid sequence of the corresponding wild-type ROS1a polypeptide,
- ii) obtaining one or more rice plants from the one or more cells obtained from step i), the one or more rice plants comprising the mutations of the endogenous ROS1a gene introduced in step i), and
- iii) detecting the mutation of the endogenous ROS1a gene and measuring the aleurone of the one or more plants of step ii), and selecting a plant of step ii) which produces grain comprising the mutation of the endogenous ROS1a gene and an aleurone that is thickened compared to aleurone from a corresponding wild-type grain thereby producing the rice plant.
A method of selecting a rice plant which produces a rice grain comprising an aleurone that is thickened compared to aleurone from a corresponding wild-type grain, the method comprising the steps of
- i) screening a population of rice plants or grain, each of which were obtained from a mutagenic treatment of progenitor rice cells, grain or plants for grain with aleruone that is thickened compared to aleurone from a corresponding wild-type grain and the presence of a mutation in a ROS1a gene, and
- ii) selecting from the population of step (i) a rice plant which comprises said mutation and which produces grain with an aleurone that is thickened compared to aleurone from a corresponding wild-type grain, thereby selecting the rice plant.
Dependencies are inferred from the claim text.