Increasing plant transformability by cytotype transfer
AssigneeSyngenta Crop Protection AG
InventorsDavid Stewart Skibbe, Sivamani Elumalai
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Abstract
A method of altering or transferring the cytotype of a plant line. In particular, transferring the cytotype of a transformation-recalcitrant plant line, e.g., a transformation-recalcitrant maize line, from a transformation-recalcitrant cytotype to a transformable cytotype so that the line becomes transformable while preserving its nuclear genome. The newly-transformable line may be produced using methods including backcrossing and/or haploid induction.
Claims14
A method of increasing transformation efficiency rate in a recalcitrant maize plant, comprising:
- a. obtaining a recalcitrant maize plant and collecting pollen therefrom;
- b. pollinating a recipient maize plant comprising normal A (“NA”) cytoplasm with pollen from the recalcitrant maize plant;
- and
- c. obtaining a progeny embryo therefrom;
- wherein the progeny embryo is grown into a progeny plant, and wherein the progeny plant comprises the NA cytoplasm of the recipient maize plant and at least the nuclear genome of the recalcitrant maize plant and wherein the progeny plant possesses a higher transformation efficiency rate than the recalcitrant maize plant, wherein the progeny plant comprising the NA cytoplasm of the recipient maize plant comprises the nucleic acid sequence of SEQ ID NO:
- 7.
A method of increasing transformation efficiency rate in a recalcitrant plant line, comprising:
- a. obtaining a recalcitrant plant and collecting pollen therefrom;
- b. pollinating a recipient plant comprising transformable cytoplasm with pollen from the recalcitrant plant;
- c. obtaining a progeny embryo therefrom;
- and
- d. optionally
- i. growing the progeny tissue into a progeny plant;
- ii. backcrossing the progeny plant using the pollen from the recalcitrant plant for at least one crossing;
- and
- e. transforming tissue from the progeny embryo;
- wherein the progeny tissue comprises the NA cytoplasm of the recipient maize plant and at least the nuclear genome of the recalcitrant plant and wherein the progeny tissue possesses a higher transformation efficiency rate than the recalcitrant plant, wherein the progeny embryo comprising the NA cytoplasm of the recipient maize plant comprises the nucleic acid sequence of SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.