Image-based assay using mark-assisted machine learning

An optical adapter for imaging a sample using a hand-held imaging device that has a light source, a camera, and a computer processor, comprising:

• an enclosure;
• a lens arranged to provide a microscopic field of view for the camera;
• a cavity within the enclosure;
• a lever within the cavity;
• a receptacle for receiving a sample holder and positioning the sample within a microscopic field of view;
• and
• an optical fiber configured to receive the light from the light source and to illuminate the receptacle,
• wherein the lever comprises at least one optical element and is configured to be moveable between a first position and a second position, wherein (i) in the first position, said imaging device is capable of imaging the sample in a bright field mode, and (ii) in the second position, said imaging device is capable of imaging the sample in a fluorescence excitation mode.

An optical adapter for imaging a sample using a hand-held imaging device that has a light source, a camera, and a computer processor, comprising:

• an enclosure;
• a cavity within the enclosure;
• a lever within the cavity;
• a receptacle for receiving a sample holder and positioning the sample within a microscopic field of view;
• and
• an optical fiber configured to receive the light from the light source and to illuminate the receptacle,
• wherein the lever comprises at least one optical element and is configured to be moveable between a first position and a second position, wherein (i) in the first position, said imaging device is capable of imaging the sample in a bright field mode, and (ii) in the second position, said imaging device is capable of imaging the sample in a fluorescence excitation mode, and
• wherein the lever comprises a first planar region extending along a first plane and a second planar region laterally displaced along a first direction from the first planar region and extending along a second plane, the first plane being disposed at a different height along a second direction from the second plane, the second direction being orthogonal to the first direction.