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US 12,119,088 B2Granted
Deep neural network-based sequencing
AssigneeIllumina, Inc.
InventorsKishore Jaganathan, Anindita Dutta, Dorna Kashefhaghighi, John Randall Gobbel, Amirali Kia
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Abstract
A system, a method and a non-transitory computer readable storage medium for base calling are described. The base calling method includes processing through a neural network first image data comprising images of clusters and their surrounding background captured by a sequencing system for one or more sequencing cycles of a sequencing run. The base calling method further includes producing a base call for one or more of the clusters of the one or more sequencing cycles of the sequencing run.
Claims20
A sequencing system, comprising:
- a receptacle coupled to a biosensor system, the biosensor system configured to comprise an array of light detectors, the biosensor system comprising a biosensor, and the biosensor comprising reaction sites configured to contain analytes;
- an illumination system configured to direct excitation light toward the biosensor and illuminate the analytes in the reaction sites, wherein at least some of the analytes provide emission signals when illuminated;
- and
- a system controller coupled to the receptacle and comprising an analysis module, the analysis module configured to:
- obtain image data from the light detectors at each of a plurality of sequencing cycles of a sequencing run, wherein the image data is derived from the emission signals detected by the light detectors and comprises images of clusters and their surrounding background,
- process the image data for each of the plurality of sequencing cycles through a neural network, and
- produce a base call for one or more of the clusters at each of the plurality of sequencing cycles.
4 more elements
Dependencies are inferred from the claim text.