Coronavirus IgG/IgM multiplexed dual path immunoassay device

A test device for determining presence of at least two different antibodies to a COVID-19 (Severe acute respiratory syndrome coronavirus-2) virus in a liquid sample, comprising:

• a first test zone configured to detect presence of the at least two different antibodies to the COVID-19 virus;
• a first sorbent strip having a first location for receiving a solution and defining a first migration path leading to the first test zone for the solution, wherein the first sorbent strip contains a first marker conjugate adapted to move with the solution along said first migration path and bind to said at least two different antibodies to the COVID-19 virus;
• and
• a second sorbent strip distinct from said first sorbent strip and having a second location for receiving the liquid sample and defining a second migration path leading to the first test zone for the liquid sample, said first and second sorbent strips touching each other at said first test zone;
• wherein the first test zone has first and second test sites located on or in at least one of said first sorbent strip and said second sorbent strip, said first test site having an immobilized first ligand binding mechanism that binds to antibodies to a first protein of the COVID-19 virus and said second test site having an immobilized second ligand binding mechanism that binds to antibodies to a second protein of the COVID-19 virus, wherein said second location is removed from said first and second test sites such that the liquid sample applied to said second location requires time to migrate to said first and second test sites and does not immediately wet said first and second test sites.