Diagnostic assay for rumen undegraded protein detection

A method for testing a food product for rumen undegraded protein, the method comprising:

• extracting a total protein content from an assay sample of the food product to provide an extraction sample, wherein the total protein content of the extraction sample comprises a rumen undegraded protein content;
• conducting gel electrophoresis on the extraction sample applied to a first gel and a second gel to separate the rumen undegraded protein content from the total protein content, thereby providing a residual total protein content;
• staining the first gel after separating the rumen undegraded protein content from the total protein content, wherein staining the first gel comprises applying a stain capable of staining glycosylated proteins in a polyacrylamide gel such that the stained glycosylated proteins correspond to the separated rumen undegraded protein content;
• staining the second gel after separating the rumen undegraded protein content from the total protein content, wherein staining the second gel comprises applying a stain capable of staining the total protein content in the extraction sample including both the separated rumen undegraded protein content and the residual total protein content in a polyacrylamide gel;
• and conducting an intensity analysis of the stained separated rumen undegraded protein content in the first gel and the stained total protein content in the second gel to determine a percentage of rumen undegraded protein in the total protein content of the food product.