Methods and kits for isolating whole cells from frozen tissues for cell fingerprinting
AssigneePeri-Nuc Labs, LLC
InventorTattym E. Shaiken
Life of this patent
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Abstract
The present disclosure relates to methods for isolating whole cells from frozen tissue samples. The present disclosure further provides kits for obtaining isolated whole cells from a frozen tissue sample. One benefit to the methods and kits can be the rapid isolation of whole cells from frozen tissues upon thawing. Another benefit to the methods and kits disclosed herein can be the rapid and efficient extraction of isolated whole cells from stroma material in frozen tissues. Another benefit to the methods and kits disclosed herein can be the isolation of whole cells from frozen tissues suitable for subsequent biochemical analysis.
Claims12
A method of isolating whole cells from a frozen tissue sample comprising:
- (a) providing a frozen tissue sample, wherein the frozen tissue sample contains whole cells and stroma material;
- (b) cutting the frozen tissue sample into two or more tissue pieces using a tissue sample cutter, wherein the tissue sample cutter has a plurality of cutting blades;
- (c) forming an extraction mixture by incubating the two or more tissue pieces in a volume of tissue sample solution (TSS) for an incubation period;
- (d) forming a whole cell sample by pipetting a first portion of the extraction mixture from the tissue sample solution into an extraction pipette, and then
- (e) pipetting a second portion of the extraction mixture from the extraction pipette into a washing solution;
- and
- (f) collecting isolated whole cells from the whole cell sample by flowing the extraction mixture through a first separation funnel into a collection vessel.
Dependencies are inferred from the claim text.