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US 12,110,557 B2Granted
Kit, device, and method for detecting ovarian tumor
AssigneesToray Industries, Inc., National Cancer Center
InventorsYuho Kida, Satoko Kozono, Hiroko Sudo, Satoshi Kondou, Junpei Kawauchi, Hitoshi Nobumasa, Takahiro Ochiya, Tomoyasu Kato
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Abstract
This application provides a kit or a device for detection of ovarian tumor, comprising a nucleic acid(s) for detecting a miRNA(s) in a sample from a subject, and a method for detecting ovarian tumor, comprising measuring the miRNA(s) in vitro.
Claims7
A method for detecting ovarian tumor, comprising:
- 1) measuring an expression level of miR-1233-5p in a sample from a subject;
- and evaluating in vitro whether or not the subject has ovarian tumor using the measured expression level;
- 2) measuring an expression level of a target gene in a sample from a subject using a kit or device comprising a nucleic acid capable of specifically binding to a polynucleotide of miR-1233-5p or to a complementary strand of the polynucleotide;
- and evaluating in vitro whether or not the subject has ovarian tumor using both of the measured expression levels and a control expression level from a healthy subject measured in the same way;
- or
- 3) Measuring an expression level of a target gene in a sample from a subject using a kit or device comprising a nucleic acid capable of specifically binding a polynucleotide of miR-1233-5p or to a complementary strand of the polynucleotide;
- and assigning the expression level of the target gene in the sample from the subject to a discriminant, which is capable of discriminatorily determining the presence or absence of ovarian tumor and is prepared with gene expression levels in a sample(s) from a subject(s) known to have ovarian tumor and a sample(s) from a subject(s) without ovarian tumor as a training sample(s), and thereby evaluating in vitro the presence or absence of ovarian tumor.
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Dependencies are inferred from the claim text.