Methods for preparing high-resolution spatial arrays
Assignee10x Genomics, Inc.
InventorFelice Alessio Bava
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Abstract
Provided herein are methods of preparing a spatial array and methods for associating specific sample analytes with spatial locations in the spatial array. Provided herein are methods for preparing a spatial array using a plurality of primers attached to a substrate to guide a plurality of features to specific locations on the spatial array. In a non-limiting example, a plurality of primers on a substrate can be used to guide a plurality of first features that include capture probes onto the substrate. A plurality of second features that are configured to hybridize to the first features and also include capture probes are added to the substrate.
Claims21
A method for preparing a spatial array, the method comprising:
- (a) providing a substrate comprising a plurality of primers attached to a surface of the substrate, wherein a primer of the plurality of primers comprises a first hybridization domain;
- (b) contacting the substrate with a plurality of first features, wherein a first feature of the plurality of first features comprises an oligonucleotide, a first capture probe, and a first bridging probe, wherein:
- (i) the oligonucleotide comprises a second hybridization domain, wherein the second hybridization domain is capable of hybridizing to the first hybridization domain;
- (ii) the first capture probe comprises a first spatial barcode and a first capture domain, wherein the first capture domain is capable of hybridizing to a first analyte from a biological sample;
- and
- (iii) the first bridging probe comprises a first bridging domain, wherein the first bridging domain is capable of hybridizing to a second bridging domain;
- and
- (c) attaching the plurality of first features to the plurality of primers by coupling the second hybridization domain to the first hybridization domain.
Dependencies are inferred from the claim text.