Metal ion-start DNA polymerase switch and isothermal polymerase amplification method using the same

A metal ion-start DNA polymerase switch comprising:

• a binding module composed of an aptamer for inhibiting DNA polymerase activity;
• a locking or unlocking module;
• and
• a catalytic module connecting between the binding module and the locking or unlocking module and composed of DNAzyme with a metal-ion recognition site,
• wherein the aptamer comprises consensus sequence motif of TQ30, which is composed of a fixed loop that directly contacts a DNA polymerase, and a programmable stem that serves as a binding stabilizer,
• wherein the metal ion-start DNA polymerase switch comprising the locking module comprises the nucleic acid sequence of SEQ ID NO:
• 1, and the metal ion-start DNA polymerase switch comprising the unlocking module comprises the nucleic acid sequence of SEQ ID NO:
• 4,
• wherein the DNAzyme is Pb2+-responsive and RNA-cleaving GR5 DNAzyme, and
• wherein the DNA polymerase is Taq polymerase.