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US 12,110,538 B2Granted
Method for detecting nucleic acid based on prokaryotic argonaute protein and application thereof
AssigneeJiaohong Biotechnology (Shanghai) Co., Ltd.
InventorsYan Feng, Guanhua Xun, Qian Liu, Yuesheng Chong
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Abstract
Provided are a method for detecting a nucleic acid based on prokaryotic Argonaute protein and an application thereof. In particular, provided is a system for detecting a target nucleic acid molecule. The system comprises guide ssDNA, a gene-editing enzyme Pyrococcus furiosus Argonaute (PfAgo), and a fluorescent reporter nucleic acid.
Claims14
A detection system for detecting a target nucleic acid molecule, wherein the system comprises:
- (a) a guide ssDNA pair;
- (b) a gene editing enzyme Pyrococcus furiosus (Pf Ago);
- and
- (c) a fluorescent reporter nucleic acid, which has a fluorescent group and a quenching group;
- wherein:
- the target nucleic acid molecule is target DNA and is cleaved by the PfAgo enzyme to produce a secondary guide ssDNA,
- the sequences of the secondary guide ssDNA and the fluorescent reporter nucleic acid are complementary;
- and
- after complementary binding between the secondary guide ssDNA and the fluorescent reporter nucleic acid, the PfAgo enzyme is guided to cleave the fluorescent reporter nucleic acid, thereby generating a detectable signal.
6 more elements
Dependencies are inferred from the claim text.