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US 12,110,533 B2Granted
Stabilisation and isolation of extracellular nucleic acids
AssigneeQiagen GmbH
InventorsMartin Horlitz, Annabelle Schubert, Markus Sprenger-Haussels
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Abstract
The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using an apoptosis inhibitor, preferably a caspase inhibitor, a hypertonic agent and/or a compound according to formula 1 as defined in the claims.
Claims14
A composition, comprising:
- (a) at least one caspase inhibitor, wherein the at least one caspase inhibitor is a pancaspase inhibitor,
- (b) at least one compound according to formula 1,
- wherein R1 is a hydrogen residue or an alkyl residue, R2 and R3 are identical or different hydrocarbon residues with a length of the carbon chain of 1-20 atoms arranged in a linear or branched manner, and R4 is an oxygen residue, and wherein the at least one compound according to formula 1 is a N,N-dialkyl-carboxylic acid amide, and
- (c) at least one chelating agent, wherein the at least one chelating agent is EDTA, wherein the composition is capable of stabilizing an extracellular nucleic acid population in a cell-containing biological sample.
1 more element
Dependencies are inferred from the claim text.