Thermostable terminal deoxynucleotidyl transferase
AssigneesIllumina, Inc., Illumina Singapore Pte. Ltd., National University of Singapore
InventorsSaurabh Rajendra Nirantar, Jasmine Puay Suan Chua, Sergio Peisajovich, Wen Shan Yew, Maybelle Darlene Kho Go
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Abstract
Disclosed herein include recombinant terminal deoxynucleotidyl transferases (TdTs). In some embodiments, the recombinant TdT comprises an amino acid sequence that is at least 80% identical to a bovine TdT, wherein the recombinant TdT comprises one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His421 in the bovine TdT.
Claims23
A recombinant terminal deoxynucleotidyl transferase (TdT) comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1, wherein the recombinant TdT comprises one or more amino acid substitution mutations at one or more positions corresponding to Glu191, Glu194, Asp242, Lys287, Met299, Thr342, and His421 in the Bos taurus TdT of SEQ ID NO:
- 12.
Dependencies are inferred from the claim text.