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US 12,110,515 B2Granted
Modified Thermococcus polymerases
AssigneeSingular Genomics Systems, Inc.
InventorsSouad Naji, Carl W. Fuller, Eli N. Glezer, Andrew Spaventa
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Abstract
Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Thermococcus sp. EP1 that exhibit improved incorporation of nucleotide analogues utilized in DNA sequences.
Claims18
A method of extending a primer, said method comprising contacting a primer hybridized to a template polynucleotide with a nucleotide and extending said primer with a polymerase by incorporating the modified nucleotide to form an extended primer, wherein the polymerase comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1;
- and further comprises:
- an alanine at amino acid position 411 or an amino acid functionally equivalent to amino acid position 411;
- a glycine at amino acid position 412 or an amino acid functionally equivalent to amino acid position 412;
- and
- a proline at amino acid position 413 or an amino acid functionally equivalent to amino acid position 413;
- and
- an alanine or glycine at amino acid position 93 or an amino acid functionally equivalent to amino acid position 93.
5 more elements
Dependencies are inferred from the claim text.