Modified polymerases for improved incorporation of nucleotide analogues
AssigneeIllumina, Inc.
InventorsCheng-Yao Chen, Erin Bomati, Molly He
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Abstract
Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3′ sugar hydroxyl, as well as methods and kits using the same.
Claims15
An altered family B archaeal polymerase comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:10, wherein the altered polymerase comprises amino acid substitution mutations at the positions functionally equivalent to Lys477 and Ala485 in the wild-type 9°N DNA polymerase amino acid sequence of SEQ ID NO:5.
A recombinant DNA polymerase comprising an amino acid sequence that is at least 80% identical to SEQ ID NO:
- 29 and comprises Val485, which recombinant DNA polymerase comprises an amino acid substitution mutation homologous to Lys477Met in the 9°N DNA polymerase amino acid sequence of SEQ ID NO:29.
A recombinant DNA polymerase comprising an amino acid sequence that is at least 80% identical to SEQ ID NO:
- 31 and comprises Val485, which recombinant DNA polymerase comprises an amino acid substitution mutation to Lys477Met in the 9°N DNA polymerase amino acid sequence of SEQ ID NO:31.
Dependencies are inferred from the claim text.