Method for differentiation of ocular cells and use thereof
AssigneeFUJIFILM Cellular Dynamics, Inc.
InventorsLucas Chase, Kyle Wallace, Benjamin Meline, Andrew Dias, Brandon Shelley, Marisa Fenn, Debjani Phillips, Matthew Sternfeld, Nathan Meyer
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Abstract
Provided herein are methods of producing a photoreceptor precursor (PRP) cell population derived from stem cells. Further provided herein are methods of using the PRP cell populations, such as for therapeutics.
Claims29
A method for producing a population of neural retinal progenitors (NRPs) comprising:
- (a) obtaining a starting population of human induced pluripotent stem cells (iPSCs);
- (b) culturing the cells in a first retinal differentiation medium (RD1) comprising a BMP inhibitor to further differentiate the cells to anterior neuroectoderm cells;
- (c) inducing retinal differentiation of the anterior neuroectoderm cells by culturing the cells in a second retinal differentiation medium (RD2) essentially free of BMP inhibitors to form retinal progenitor cells (RPCs);
- and
- (d) culturing the RPCs in a retinal maturation (RM) medium to produce NRPs.
A composition comprising an NRP population derived from iPSCs, wherein at least 90% of the cells in the NRP population express PAX6, at least 90% of the cells in the NRP population express PMEL17, and at least 70% of the cells in the NRP population express VSX2.
Dependencies are inferred from the claim text.