Back to results
US 12,110,314 B2Granted
PepCon proteomics standards and methods of use
AssigneeLawrence Livermore National Security, LLC
InventorsMathew Gerald Lyman, Deon S. Anex, Bonnee Rubinfeld
Life of this patent
Estimating the term…
Abstract
Described are methods, compositions, and devices for a concatemeric protein standard that behaves as a protein but transforms into single peptides upon digestion, which is optimized to function as a non-obtrusive process control for mass spectrometry analysis.
Claims18
A method of using a qualitative control for protein mass spectrometry, comprising:
- (a) generating an analysis sample by combining a protein sample with a peptide concatemer (“PepCon”), wherein the PepCon comprises two or more copies of a peptide linked by a cleavage site and the peptide comprises the sequence set forth in SEQ ID NO. 7 or a variant thereof which is at least 80% homologous to the SEQ ID NO. 7;
- and
- (b) digesting the analysis sample with an agent capable of cleaving at the cleavage site.
A method of performing protein mass spectrometry, the method comprising:
- preparing an analysis sample containing a protein sample and a peptide concatemer (“PepCon”) for mass spectrometry, wherein the PepCon includes a protein structure having two or more copies of a peptide linked together and the peptide comprises the sequence set forth in SEQ ID NO. 7 or a variant thereof which is at least 80% homologous to the SEQ ID NO. 7, and wherein preparing the analysis sample includes digesting the PepCon to produce at least two unlinked copies of the peptide;
- and
- subjecting the analysis sample to mass spectrometry, wherein the at least two peptides act as a qualitative control for mass spectrometry analysis.
A method of performing protein mass spectrometry using a qualitative control, the method comprising:
- subjecting an analysis sample containing a protein sample and two or more copies of a control peptide to mass spectrometry, wherein the two or more copies of the control peptide include a common sequence and were sourced from a peptide concatemer (“PepCon”) containing the two or more copies of the control peptide linked at a cleavage site and the control peptide comprises the sequence set forth in SEQ ID NO. 7 or a variant thereof which is at least 80% homologous to the SEQ ID NO. 7.
Dependencies are inferred from the claim text.