Variant of nitrous oxide reductase protein and method of reducing concentration of nitrous oxide in sample
AssigneeSamsung Electronics Co., Ltd.
InventorsWoo Yong Shim, Seung Hoon Song, Jae-Young Kim, Yu Kyung Jung
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Abstract
Provided are recombinant microorganisms having a foreign gene encoding a variant of a nitrous oxide reductase protein, a composition comprising the recombinant microorganism or the variant of a nitrous oxide reductase protein for use in removing nitrous oxide in a sample, a variant of a nitrous oxide reductase protein, and a polynucleotide encoding the variant.
Claims19
A recombinant microorganism comprising a foreign gene encoding a nitrous oxide reductase enzyme, wherein the enzyme is nitrous oxide reductase NosZ having at least 85% amino acid sequence identity to SEQ ID NO:
- 1, and wherein the enzyme comprises an amino acid alteration of an amino acid residue corresponding to position H78 of the amino acid sequence of SEQ ID NO:
- 1, wherein the microorganism belongs to the genus Escherichia.
A composition comprising a recombinant microorganism for reducing a concentration of nitrous oxide in a sample as compared to a sample without the recombinant microorganism, the recombinant microorganism comprising a foreign gene encoding a nitrous oxide reductase enzyme, wherein the enzyme is nitrous oxide reductase NosZ having at least a 85% amino acid sequence identity to SEQ ID NO:
- 1, and wherein the enzyme has an amino acid alteration of an amino acid residue corresponding to position H78 of the amino acid sequence of SEQ ID NO:
- 1, wherein the microorganism belongs to the genus Escherichia.
A polynucleotide encoding a nitrous oxide reductase enzyme, wherein the enzyme is nitrous oxide reductase NosZ having at least 85% amino acid sequence identity to SEQ ID NO:
- 1, and wherein the enzyme comprises an amino acid alteration of the amino acid residue corresponding to position H78 of the amino acid sequence of SEQ ID NO:
- 1 and the amino acid alteration is a substitution of the amino acid residue corresponding to position H78 with A, M, N, E, P, F, I, or C.
A method of reducing a concentration of nitrous oxide in a sample comprising nitrous oxide, the method comprising contacting a sample comprising nitrous oxide with a recombinant microorganism comprising a foreign gene encoding a nitrous oxide reductase protein, wherein the protein is nitrous oxide reductase NosZ having at least 85% amino acid sequence identity to SEQ ID NO:1, and—wherein the enzyme comprises an amino acid alteration of an amino acid residue corresponding to position H78 of the amino acid sequence of SEQ ID NO:
- 1 wherein the microorganism belongs to the genus Escherichia.
Dependencies are inferred from the claim text.