Scaffolds from self-assembling tetrapeptides support 3D spreading, osteogenic differentiation and angiogenesis of mesenchymal stem cells
AssigneeKing Abdullah University of Science and Technology
InventorsCharlotte A. E. Hauser, Salwa Alshehri, Hepi H. Susapto
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Abstract
The present disclosure relates generally to an osteo-tissue graft capable of promoting bone tissue growth and regeneration, comprising at least one self-assemble peptide and mesenchymal stem cells (MSCs) in accordance with the present invention and a method of preparing such an osteo-tissue graft. The grafts are suitable for treatment of bone disorder or damages through tissue engineering, cellular replacement therapies as well as other applications.
Claims16
A 3-dimensional osteo-tissue graft comprising:
- live mesenchymal stem cells (MSCs);
- and
- ultrashort self-assembling peptide scaffolds,
- wherein the stiffness of the peptide scaffolds is 16-25 kPa,
- wherein at least one osteogenic marker selected from the group consisting of bone morphogenetic protein (BMP-2) bone sialoprotein 2 (IBSP), osteopontin (OPN), osterix (OSX), RUNX2, osteocalcin and alkaline phosphatase (ALP), is expressed.
A method to create a 3-dimensional osteo-tissue graft comprising:
- suspending mesenchymal stem cells (MSCs) in buffer solution;
- dissolving an ultrashort self-assembling peptide in buffer solution;
- loading a 3D bioprinter with the suspended MSCs and peptide solution;
- creating the 3-dimensional osteo-tissue graft using the 3D bioprinter or manually;
- incubating the 3-dimensional osteo-tissue graft for 5 min at 37° C.;
- culturing the 3-dimensional osteo-tissue graft in complete media;
- and
- 3D culturing the MSCs by keeping the 3-dimensional osteo-tissue graft in osteogenic induction media or basal stem cell growth media,
- wherein the 3-dimensional osteo-tissue graft comprises live MSCs and at least one ultrashort self-assembling peptide scaffolds,
- wherein the stiffness of the peptide scaffolds is 20-25 kPa.
Dependencies are inferred from the claim text.