Compositions for treating wounds
OwnerSamuel E. Lynch, Leslie Wisner-Lynchinventors
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Abstract
Novel compositions for treating wounds and promoting the healing thereof are described, including composition containing novel combinations of a carrier and recombinant platelet derived growth factor having fewer isoforms and enhanced biostability. Methods of treating wounds with novel therapeutic composition using doing procedures leading to effective results with a minimal number of treatment applications are also described.
Claims9
A lyophilized therapeutic composition consisting essentially of recombinant human platelet-derived growth factor (rhPDGF) B chain homodimer (rhPDGF-BB), incorporated into a biocompatible matrix, wherein the lyophilized therapeutic composition is formed from a rhPDGF-BB solution disposed in a porous biocompatible matrix,
- wherein:
- (a) the rhPDGF-BB solution contains between about 0.05 mg/ml to about 5 mg/ml of rhPDGF-BB in a buffer;
- (b) the porous biocompatible matrix consists of a natural polymer selected from the group consisting of collagen, gelatin, fibrin, alginate, cellulose, chitosan and fibronectin;
- (c) the porous biocompatible matrix includes an open porous structure that allows for cell attachment and ingrowth into said pores, wherein the pores have a pore size distribution of between 50 μm and 1000 μm, or an average pore size of between 50 μm and 500 μm, and wherein said pores entrap at least 50% of said rhPDGF-BB within said pores;
- (d) the ratio of the rhPDGF-BB solution to the matrix is between about 0.05 ml/cm3 to about 5 ml/cm3;
- (e) upon wetting with a physiologic solution, the lyophilized therapeutic composition yields a ratio of the rhPDGF-BB to the matrix that is between about 75 μg PDGF/cm3 of matrix to about 225 μg PDGF/cm3 of matrix;
- and
- (f) the lyophilized therapeutic composition is biostable and retains at least 80% of its bioactivity when stored for at least six months.
Dependencies are inferred from the claim text.