Back to results
US 12,109,310 B2Granted
Exosome extracellular vesicles and methods of use
AssigneesHadi Valadi, Astraeneca AB
InventorsHadi Valadi, Lennart Lindfors
Life of this patent
Estimating the term…
Abstract
Exosomes comprising a modified RNA are disclosed. Aspects of the disclosure further relate to methods and compositions for using exosomes comprising a modified RNA. In certain aspects, the exosomes disclosed herein may be useful in delivering a modified RNA to a cell. In certain aspects, the exosomes disclosed herein may be useful in treating or preventing a disorder in a subject. Methods and compositions for producing the disclosed exosomes are also provided.
Claims16
An isolated exosome comprising a modified ribonucleic acid (RNA) prepared by a process comprising:
- (a) providing one or more lipid nanoparticles (LNP) comprising the modified RNA;
- (b) contacting one or more cells with the LNP under conditions that allow LNP uptake by the one or more cells;
- and
- (c) isolating exosomes produced by the one or more cells, wherein at least one isolated exosome comprises the modified RNA;
- wherein the exosome comprises an ionizable lipid:modified RNA nucleotides molar ratio of about 1:1 to about 3:1, or less than about 1:1.
2 more elements
Dependencies are inferred from the claim text.