Method for preparing enzyme-treated Zizania latifolia Turcz. extract having increased tricin content, and composition for whitening, wrinkle reduction, anti-inflammation, anti-allergy and moisturization, prepared thereby

A method of enhancing skin activities, comprising administering an effective amount of a health functional food comprising an enzyme-treated Zizania latifolia Turcz. extract prepared by the method comprising the steps of:

• (1) adding water to Zizania latifolia Turcz. to produce a solution and heating for a sufficient duration of time;
• (2) cooling heated solution and treating with one or more enzyme(s) selected from the group consisting of:
• α-amylase, β-galactosidase, xylanase, lactase, lipase, tannase, cellulase, hemicellulase, pectinase and β-glucosidase, to produce an enzyme-treated solution;
• (3) filtering the enzyme-treated solution to obtain a primary extract and a remaining residue;
• (4) extracting the remaining residue after filtering in step (3) with any solvent selected from the group consisting of:
• water, lower alcohols of C1 to C4 and mixtures thereof to obtain a secondary extract;
• and
• (5) mixing the primary and secondary extracts and concentrating or drying to produce the enzyme-treated Zizania latifolia Turcz. extract;
• wherein the enzyme-treated Zizania latifolia Turcz. extract contains 0.01% to 90% by weight of tricin.