Polymer nanoparticle and DNA nanostructure compositions and methods for non-viral delivery

A method of preparing a library of RAFT block copolymers comprising:

• i. providing an array of reaction mixtures in a multiwell plate, wherein each well comprises a mixture of a first chain transfer agent, a first initiator, one or more monomer units, and optionally a solvent or solvent mixture;
• ii. reacting the mixture in each well under conditions that promote RAFT copolymerization to provide a series of first block copolymers in the wells of the multiwell plate;
• iii. quenching the reactions in the wells of the multiwell plate;
• iv. purifying the first block copolymer in each well of the multiwell plate;
• v. optionally characterizing the first block copolymer in each well of the multiwell plate;
• vi. optionally purifying the first block copolymer in each well of the multiwell plate;
• vii. contacting to the first block copolymer in each well of the multiwell plate with a second array of reaction mixtures comprising a second chain transfer agent, a second initiator, and one or more monomer units, and optionally a solvent or solvent mixture;
• viii. reacting the components of step (vii) under conditions that promote RAFT copolymerization to provide a series of RAFT block copolymers in the wells of the multiwell plate;
• ix. quenching the reactions in the wells of the multiwell plate;
• x. purifying the RAFT block copolymer in each well of the multiwell plate;
• and
• xi. optionally characterizing the RAFT block copolymer in each well of the multiwell plate.