Synthesis of antimicrobial carbon dots and uses thereof
AssigneeB.G. Negev Technologies and Applications Ltd., At Ben-Gurion University
InventorsRaz Jelinek, Sagarika Bhattacharya, Gil Otis
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Abstract
The present invention relates to antibacterial carbon dots, having aminoguanidine functionality on their outermost surface. The invention further relates to the synthesis of said antibacterial carbon dots, and to the uses of these carbon dots for inhibiting a biofilm formation in the presence of said carbon dots and as fluorescent labeling markers for specific bacteria.
Claims22
Antimicrobial carbon-dots (C-dots) having at least one aminoguanidine functional group on their outer surface, wherein said C-dots are characterized in having a maximal emission at a wavelength of about 480 nm upon excitation at a wavelength of 390 nm, and wherein said antimicrobial C-dots inhibit the growth of Pseudomonas aeruginosa.
A method for inhibiting the formation of a biofilm and/or disrupting the propagation of a biofilm comprising contacting antimicrobial C-dots having at least one aminoguanidine functional group on their outer surface with biofilm forming bacterial cells.
A method for selective bacterial cell labeling, comprising the steps of (A) contacting bacterial cells sought to be labeled with fluorescent C-dots having at least one aminoguanidine functional group on their outer surface at a concentration range of between about 0.1 mg/ml to about 1 mg/ml;
- and (B) removing excess C-dots;
- thereby providing precise cell labeling.
A method for preparing antimicrobial C-dots having at least one aminoguanidine functional group on their outer surface, wherein said method comprises a step of reacting a carbonaceous molecule with an aminoguanidine precursor in aqueous media under heating, wherein a mass ratio between said aminoguanidine precursor and said carbonaceous molecule is between about 2.5:1 to about 1.5:1.
Dependencies are inferred from the claim text.