Back to results
US 12,108,745 B2Granted
Rat model of IgA nephropathy induced with a multimeric recombinant IgA fragment
AssigneeNorthwestern University
InventorsJing Jin, Xinfang Xie
Life of this patent
Estimating the term…
Abstract
Recombinant poly-IgA oligomers that form high-order oligomers resembling poly-IgA of IgA nephropathy are provided. Injection of recombinant IgA oligomers in an animal model produces prominent renal glomerular mesangial deposition of recombinant poly IgA oligomer, as in IgA nephropathy patients. Thus, producing a model of IgAN pathology that is able to provide screening and evaluation of therapeutic drugs and diagnostic tests.
Claims8
A method of inducing IgA nephropathy in a subject comprising:
- providing a polymeric complex of unglycosylated IgA CH2-CH3 oligomeric fragments, wherein each of the IgA CH2-CH3 oligomeric fragments comprises a biotin moiety at the N-terminus of the IgA CH2-CH3 and the polymeric complex is formed by the addition of streptavidin to a mixture of biotinylated IgA CH2-CH3 oligomeric fragments;
- and
- daily, intravenous administering to a subject the polymeric complex of unglycosylated IgA CH2-CH3 oligomeric fragments,
- wherein the daily administration of the polymeric complex to the subject for a period of twelve consecutive days induces in the subject kidney glomerulus mesangial deposition of the polymeric complex of unglycosylated IgA fragments and induction of renal and systemic responses toward clearance of the polymeric complex from the kidney, the deposition of IgA polymers and renal and systemic responses toward clearance of IgA polymers being indicators of a IgA nephropathy,
- wherein the subject is a rodent.
2 more elements
A method for producing an IgA nephropathy animal model comprising:
- providing a polymeric complex of unglycosylated IgA CH2-CH3 oligomeric fragments, wherein each of the IgA CH2-CH3 oligomeric fragments comprises a biotin moiety at the N-terminus of the IgA CH2-CH3 and the polymeric complex is formed by the addition of streptavidin to a mixture of biotinylated IgA CH2-CH3 oligomeric fragments and
- daily, intravenous administering to a subject the polymeric complex of unglycosylated IgA CH2-CH3 oligomeric fragments,
- wherein the daily administration of the polymeric complex to the subject for a period of twelve consecutive days induces in the animal kidney glomerulus mesangial deposition of the polymeric complex of unglycosylated IgA fragments thus inducing renal and systemic responses toward clearance of the polymeric complex from the kidney, thereby producing an animal model of IgA nephropathy,
- wherein the animal model is a rodent.
1 more element
Dependencies are inferred from the claim text.