Methods for creating doubled haploid plants
AssigneeMonsanto Technology LLC
InventorsFenggao Dong, Michael Graham, Huachun Larue, Daniel Ovadya
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Abstract
Provided are methods for increasing the efficiency of creating doubled haploid plants by increasing the number of chances of forming a double haploid seed through treatment of a monocot plant with a plant growth regulator. In certain embodiments, maize plants are produced that comprise multiple co-dominant ears. Also provided are plants comprising the potential to generate increased numbers of doubled haploid offspring.
Claims18
A method of producing co-dominant ears on a haploid maize plant, the method comprising contacting the haploid maize plant with a plant growth regulator, wherein the haploid maize plant produces at least two co-dominant ears, wherein each of the at least two co-dominant ears are receptive to pollination during pollen release from the haploid maize plant,
- wherein plant growth regulator is paclobutrazol.
A method of improving the number of DH1 seeds harvested from a DH0 maize plant, the method comprising contacting the DH0 maize plant with a plant growth regulator at developmental stage V2, V3, V4, V5, V6, V7, V8, V9, V10, V11, or V12* and contacting the DH0 maize plant with a chromosome doubling agent at any stage of its life cycle, to produce a DH0 maize plant that produces at least one DH1 maize seed and at least two co-dominant ears,
- wherein each of the at least two co-dominant ears are receptive to pollination during pollen release from the DH0 maize plant,
- wherein plant growth regulator is paclobutrazol.
Dependencies are inferred from the claim text.