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US 12,091,698 B2Granted
Compositions and methods for RNA synthesis
AssigneeLineaRx, Inc.
InventorsBrendan Hussey, Lai Him Chung
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Abstract
Provided herein are compositions, methods, devices, and systems for highly accurate and pure RNA synthesis. Also provided herein are nucleic acid libraries comprising RNAs generated by using devices, compositions and methods disclosed herein.
Claims15
A composition comprising a modified polypeptide, wherein the modified polypeptide comprises:
- a purified T7 RNA polymerase, a functional fragment, or a functional variant thereof;
- and
- a purified nucleic acid binding protein,
- wherein the purified T7 RNA polymerase and the purified nucleic acid binding protein are heterologous,
- wherein the purified T7 RNA polymerase, the functional fragment, or the functional variant thereof,
- and the purified nucleic acid binding protein are linked,
- wherein the purified nucleic acid binding protein is an avidin, and
- wherein the purified nucleic acid binding protein is linked to the C-terminus or N-terminus of the purified RNA polymerase, the functional fragment, or the functional variant thereof by fusion.
5 more elements
Dependencies are inferred from the claim text.