Immunogenic composition
AssigneeGlaxosmithkline Biologicals SA
InventorsChristiane Marie-Paule Simone Jeanne Feron, Sandra Giannini
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Abstract
The present invention discloses an engineered bacteriophage capable of binding to a commensal bacterium and inserting its genome polynucleotide into the commensal bacterium, but incapable of producing progeny, incapable of carrying out a lysogenic cycle and incapable of carrying out a lytic cycle within the commensal bacterium, wherein the engineered bacteriophage comprises a genome polynucleotide including at least one gene encoding at least one heterologous antigen(s) under the control of a promoter.
Claims17
An engineered bacteriophage capable of binding to a commensal bacterium and inserting its genome polynucleotide into the commensal bacterium, but incapable of producing progeny, incapable of carrying out a lysogenic cycle and incapable of carrying out a lytic cycle within the commensal bacterium, wherein the engineered bacteriophage comprises a genome polynucleotide including at least one gene encoding at least one heterologous antigen(s) under the control of a promoter and the genome polynucleotide is engineered to delete all genes encoding lytic machinery proteins.
An engineered bacteriophage genome polynucleotide comprising a heterologous antigen gene encoding a heterologous antigen under the control of a promoter wherein the engineered bacteriophage genome polynucleotide is engineered to delete at least one gene encoding a capsid protein, and all genes encoding lytic machinery proteins, and optionally at least one gene encoding a lysogeny machinery protein, wherein when the engineered bacteriophage genome polynucleotide is packaged into a bacteriophage, the resulting bacteriophage is capable of binding to a commensal bacterium and inserting the engineered bacteriophage genome polynucleotide into the commensal bacterium, but is incapable of producing progeny, incapable of carrying out a lysogenic cycle and incapable of carrying out the lytic cycle within a commensal bacterium.
Dependencies are inferred from the claim text.