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US 12,065,668 B2Granted
RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
AssigneeThe General Hospital Corporation
InventorsJ. Keith Joung, Morgan Maeder
Life of this patent
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Abstract
Methods and constructs for RNA-guided targeting of heterologous functional domains such as transcriptional activators to specific genomic loci.
Claims7
A method of silencing or repressing expression of a target gene in a cell, the method comprising expressing
- (i) a fusion protein comprising catalytically inactive CRISPR associated 9 (dCas9) protein linked to a heterologous functional domain, wherein the heterologous functional domain is a Heterochromatin Protein 1 (HP1), and
- (ii) one or more guide RNAs directed to the target gene.
Dependencies are inferred from the claim text.