Display substrate, method of manufacturing the same, display device and display panel

A display substrate, comprising:

• a base substrate;
• a first conductive pattern on the base substrate;
• an organic layer on a side of the first conductive pattern away from the base substrate;
• and
• a second conductive layer on a side of the organic layer away from the base substrate;
• wherein
• the display substrate comprises a pixel region and a bonding region, and the first conductive pattern is in the bonding region of the display substrate;
• a via hole penetrating the organic layer is arranged in the organic layer along a direction perpendicular to the base substrate, a position of the via hole corresponds to a position of the first conductive pattern, and the second conductive layer is electrically connected to the first conductive pattern through the via hole;
• the display substrate further comprises a filling structure for filling the via hole, the filling structure is located on a side of the second conductive layer away from the base substrate, an orthogonal projection of the filling structure onto the base substrate is within an orthogonal projection of the via hole onto the base substrate, a distance difference between a distance from a surface of the filling structure on a side away from the base substrate to the base substrate and a distance from a surface of the organic layer on a side away from the base substrate to the base substrate is smaller than a preset threshold.