System for single-molecule isolation for cell populations and single cells, and methods and uses thereof
AssigneeThe Hong Kong University of Science and Technology
InventorsPingbo Huang, Qirui Zhao
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Abstract
The invention relates to the systems and methods for isolating single cells, capturing selected prey biomolecules within said cells, and detecting said prey biomolecules using a sensitive assay method. Also described herein are functionalized substrates and compositions for enabling the low limit of detection of selected prey biomolecules of selected cells.
Claims27
A system comprising a glass substrate comprising a plurality of micron-sized features, a plurality of primary antibodies against a plurality of bait molecules, the plurality of bait molecules, a plurality of target prey biomolecules, and a plurality of primary antibodies against said plurality of target prey biomolecules, wherein the plurality of primary antibodies against the plurality of bait molecules coat a plurality of beads.
Dependencies are inferred from the claim text.