Optical bio-sensing device for bio-material analysis using upconversion nanoparticles, and bio-material quantitative analysis using the same

An optical bio-sensing device for bio-material analysis using upconversion nanoparticles, the optical bio-sensing device comprising:

• a transparent substrate covering a top of a space accommodating therein a sample containing a target bio-material;
• a signal converter fixed to the transparent substrate, and including the upconversion nanoparticles for receiving incident light and emitting converted light of a wavelength shorter than a wavelength of the incident light;
• a signal reflector including retroreflection particles bindable to the signal converter via the target bio-material, wherein the retroreflection particles retroreflect the converted light;
• a light source for irradiating the incident light to the signal converter;
• and
• a light receiver for receiving light retroreflected from the signal reflector,
• wherein the target bio-material is in contact with the upconversion nanoparticles of the signal converter and is bonded to the upconversion nanoparticles of the signal converter, and
• where the target bio-material is in contact with the retroreflection particles of the signal reflector and is bonded to the retroreflection particles of the signal reflector.