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US 12,006,542 B2Granted
Force-modulated hybridization for visualizing nucleic acid length and function
AssigneeUniversity of Houston System
InventorsShoujun Xu, Qiongzheng Hu, Yuhong Wang
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Abstract
Embodiments of the present disclosure pertain to methods of utilizing force-modulated hybridization to determine the length of an analyte strand, to determine an unknown nucleic acid sequence, or to determine the binding of a nucleotide to an active agent. Additional embodiments of the present disclosure pertain to sample holder devices and methods of utilizing such devices. Further embodiments of the present disclosure pertain to detection devices.
Claims11
A method of determining the length of an analyte strand, comprising:
- (a) incubating a magnetically labeled oligonucleotide strand, the analyte strand, and one of a series of oligonucleotide ruler strands to form a mixture,
- wherein the magnetically labeled oligonucleotide strand is complementary in sequence to the analyte strand,
- wherein the series of oligonucleotide ruler strands are complementary in sequence to the magnetically labeled oligonucleotide strand and comprise different lengths, and
- wherein either the analyte strand or the oligonucleotide ruler strands are labeled with at least one label;
- (b) transferring the mixture to a surface functionalized to couple with the at least one label;
- (c) applying a mechanical force to the mixture;
- and
- (d) inspecting the surface for immobilized particles,
- wherein if the analyte strand is labeled, then the longest oligonucleotide ruler strand producing immobilized particles on the surface represents the length of the analyte strand, and
- wherein if the oligonucleotide ruler strands are labeled, then the longest oligonucleotide ruler strand not producing immobilized particles represents the length of the analyte strand;
- and
- (e) wherein a different oligonucleotide ruler strand is used to repeat steps (a)-(d) until the length of the analyte strand is determined.
9 more elements
Dependencies are inferred from the claim text.