Production of RNA by yeasts with recombinant pseudo-viral particles

A method for the recombinant production of messenger RNA (mRNA) or non-coding long-chain RNA (lncRNA), which comprises:

• culturing yeast cells, which have been genetically modified to produce recombinant pseudo-viral particles which comprise said mRNA or lncRNA, and
• collecting the mRNA or lncRNA which is contained in the virus-like particles thus formed,
• wherein the yeast cells are cells of a yeast which is free of Ty1 retrotransposon or in which any Ty1 retrotransposon has been genetically modified to not produce a Ty1 retrosome,
• wherein the genetic modifications made to these yeast cells to produce said virus-like particles comprise transfection of these yeast cells by
• i. a nucleic acid comprising a first nucleotide sequence, and
• ii. a nucleic acid comprising a second nucleotide sequence,
• wherein:
• the nucleic acid of i. and the nucleic acid of ii. are carried by the same molecule, or by two distinct or separate molecules,
• the first nucleotide sequence comprises a DNA sequence which encodes the expression of the Gag protein of a yeast Ty1 retrotransposon,
• the second nucleotide sequence comprises a DNA sequence having no reading frame, the first codon of which would be the ATG codon, whose RNA transcript contains two parts:
• a. the sequence of said mRNA or lncRNA linked to
• b. the gag addressing sequence comprising the sequence of SEQ ID NO:
• 16,
• the nucleic acid of ii. does not comprise a DNA sequence whose RNA transcript would be the complete RNA sequence of said Gag protein of a yeast Ty1 retrotransposon,
• the Gag protein of a yeast Ty retrotransposon in both nucleic acids is the same Gag protein of a yeast Ty1 retrotransposon, and
• the nucleic acid of i. and the nucleic acid of ii. do not comprise a sequence encoding the reverse transcriptase of a yeast Ty1 retrotransposon, the nucleic acid of i. and the nucleic acid of ii. thereby encoding the formation, in said yeast cells, of virus-like particles which are formed by the Gag protein translated from the first nucleotide sequence, and which encapsulate said mRNA or lncRNA transcribed from the second nucleotide sequence.