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US 12,006,516 B2Granted
Differentiation of pancreatic endocrine cells
AssigneeVertex Pharmaceuticals Incorporated
InventorsGeorge Harb, Chunhi Xie
Life of this patent
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Abstract
Disclosed herein are compositions and methods related to differentiation of stem cells into pancreatic endocrine cells. In some aspects, the methods provided herein relate to generation of pancreatic β cell, α cell, δ cells, and EC cells in vitro. In some aspects, the disclosure provides pharmaceutical compositions including the cells generated according to the methods disclosed herein, as well as methods of treatment making use thereof.
Claims30
A method of administering a composition to a Type 1 Diabetes subject, wherein the composition comprises a cell population, wherein:
- (i) the cell population comprises cells that express C-peptide but do not express VMAT1;
- (ii) at least 80% of the cells in the cell population express ISL1;
- (iii) at most 35% of the cells in the cell population express VMAT1;
- and
- (iv) less than 6% of the cells in the population are NKX6.1-negative, ISL1-negative cells.
2 more elements
Dependencies are inferred from the claim text.