Back to results
US 12,006,512 B2Granted
Method for producing CD8alpha+beta+ cytotoxic t cells
AssigneeKyoto University
InventorsShin Kaneko, Yohei Kawai
Life of this patent
Estimating the term…
Abstract
A method efficiently produces cytotoxic T lymphocytes having intrinsic properties of lymphocytes of the acquired immune system suitable for cellular immunotherapy. The method includes culturing CD4/CD8 double-positive T cells in a medium containing IL-7 and a T-cell receptor activator, to induce CD8α+β+ cytotoxic T lymphocytes.
Claims15
A method of producing CD8α+β+ cytotoxic T lymphocytes, the method comprising:
- (a) culturing CD4/CD8 double-positive T cells in the presence of IL (interleukin)-7 and a T-cell receptor activator, wherein the T-cell receptor activator is an anti-CD3 antibody, thereby producing CD8α+β+ cytotoxic T lymphocytes, and
- (b) culturing the cells obtained in step (a) in the presence of IL-7, but in the absence of a T-cell receptor activator, wherein the culture (b) is carried out using a culture vessel containing a fibronectin fragment and/or a Notch ligand.
Dependencies are inferred from the claim text.