Perfusion culturing methods and uses thereof

A multi-well cell culture plate system, comprising:

• a unitary support plate comprising a first surface comprising a plurality of apertures;
• a plurality of culture vessels disposed within the unitary support plate and configured to house cell cultures having a volume of between about 200 μL to about 18 mL in volume, wherein each aperture is paired with and defines an opening into each culture vessel and wherein each culture vessel further comprises at least one port configured to accommodate a flow of fluid into and/or out of the culture vessel, each port in fluid connection with its own conduit, the conduit disposed within the unitary support plate and defines its own fluid flow passage connecting the port and a second surface of the unitary support plate, wherein the second surface of the unitary support plate is opposite of the first surface of the unitary support plate, and each fluid flow passage do not intersect in the unitary support plate.