Methods and products for expressing proteins in cells
AssigneeFactor Bioscience Inc.
InventorsMatthew Angel, Christopher Rohde
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Abstract
The present invention relates in part to nucleic acids encoding proteins, therapeutics comprising nucleic acids encoding proteins, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods and products for altering the DNA sequence of a cell are described, as are methods and products for inducing cells to express proteins using synthetic RNA molecules. Therapeutics comprising nucleic acids encoding gene-editing proteins are also described.
Claims9
A composition comprising a nucleic acid molecule encoding a gene-editing protein, the gene-editing protein comprising:
- (a) a DNA-binding domain, and
- (b) a nuclease domain, wherein:
- (i) the DNA-binding domain comprises a plurality of repeat sequences and at least one of the repeat sequences comprises the amino acid sequence LTPvQVVAIAwxyzGHGG (SEQ ID NO:
- 75) and is 36 or 37 amino acids long, wherein:
- “v” is Q, D or E,
- “w” is S or N,
- “x” is N, H, or I,
- “y” is any amino acid,
- “z” is GGKQALETVQRLLPVLCQD
- (SEQ ID NO:
- 670)
- or
- GGKQALETVQRLLPVLCQA,
- (SEQ ID NO:
- 671)
- and
- (ii) the nuclease domain comprises a catalytic domain of a nuclease.
Dependencies are inferred from the claim text.