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AssigneeLong Chen
InventorsLong Chen, Fake Yang, Fukun Chen, Huatao Niu, Yongzhu Pu, Jindan Li, Conghui Yang, Dongxue Chen
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An application of SCFV protein in preparation of a CAR gene expression vector is disclosed. The protein sequence of the SCFV protein is shown in SEQ ID No. 1. The 1st˜21st amino acids are the extracellular signal peptide amino acid sequences. The 22nd˜31st amino acids are Flag amino acid sequences. And the remaining sequences are CXCR4 scfv sequences. An application of the gene sequence encoding the SCFV protein in preparation of a CAR gene expression vector is disclosed, and the gene sequence is shown in SEQ ID No.2. A CAR gene expression vector and a CAR-T cell are disclosed. The expression vector is plent-EF1a-Flag-CXCR4 CAR, and CAR-T cells are used in the preparation of a drug for a targeted therapy of tumor cells with high expression of CXCR4.
A method of preparing a chimeric antigen receptor (CAR) gene expression vector, comprising ligating a nucleic acid that encodes a protein comprising the ScFv having the amino acid sequence set forth in SEQ ID NO:
A chimeric antigen receptor (CAR) gene expression vector, which is a lentivirus vector comprising the nucleic acid sequence set forth in SEQ ID NO:
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