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US 12,005,454 B2Granted
Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
Assignee10x Genomics, Inc.
InventorsRajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald A. Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
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Abstract
The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
Claims14
A microfluidic system, comprising:
- a first fluid source fluidly connected to a first channel and comprising a first fluid that comprises an aqueous fluid and a plurality of microcapsules;
- an agitating component disposed to agitate the first fluid source;
- a second fluid source fluidly connected to a second channel and comprising a second fluid that is immiscible with the aqueous fluid;
- a droplet generation junction fluidly connected to a third channel;
- and
- a flow control system that comprises a first independently controlled pressure source that causes the first fluid from the first fluid source to flow along the first channel and a second independently controlled pressure source that causes the second fluid from the second fluid source to flow along the second channel, such that the microcapsules and the second fluid meet at the droplet generation junction to generate a plurality of droplets comprising the microcapsules from the plurality of microcapsules, which plurality of droplets flow along the third channel.
3 more elements
A microfluidic system, comprising:
- a first fluid source fluidly connected to a first channel and comprising a first fluid that comprises an aqueous fluid and a plurality of microcapsules;
- an agitating component disposed to agitate the first fluid source;
- a second fluid source fluidly connected to a second channel and comprising a second fluid that is immiscible with the aqueous fluid;
- a droplet generation junction fluidly connected to a third channel;
- and
- a flow control system that:
- (i) causes the first fluid from the first fluid source to flow along the first channel, and
- (ii) causes the second fluid from the second fluid source to flow along the second channel, such that the microcapsules and the second fluid meet at the droplet generation junction to generate a plurality of droplets comprising the microcapsules from the plurality of microcapsules, which plurality of droplets flow along the third channel.
5 more elements
Dependencies are inferred from the claim text.